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Fig. 2. Growth differentiation factor 15 <t>(GDF15)</t> had cardioprotective effects in HFpEF. (A) Silencing GDF15 further exacerbates
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Fig. 2. Growth differentiation factor 15 <t>(GDF15)</t> had cardioprotective effects in HFpEF. (A) Silencing GDF15 further exacerbates
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Fig. 2. Growth differentiation factor 15 (GDF15) had cardioprotective effects in HFpEF. (A) Silencing GDF15 further exacerbates

Journal: Frontiers in Bioscience-Landmark

Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

doi: 10.31083/fbl26857

Figure Lengend Snippet: Fig. 2. Growth differentiation factor 15 (GDF15) had cardioprotective effects in HFpEF. (A) Silencing GDF15 further exacerbates

Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

Techniques:

Fig. 3. GDF15 inhibited cardiac fibrosis in HFpEF. (A) Masson’s trichrome staining for collagen deposition in the cardiac tissue

Journal: Frontiers in Bioscience-Landmark

Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

doi: 10.31083/fbl26857

Figure Lengend Snippet: Fig. 3. GDF15 inhibited cardiac fibrosis in HFpEF. (A) Masson’s trichrome staining for collagen deposition in the cardiac tissue

Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

Techniques: Staining

Fig. 4. GDF15 decreased oxidative stress in HFpEF. Expression of oxidative stress-associated biomarkers, Myeloperoxidase (MPO) and oxidized low-density lipoprotein (ox-LDL). The original western blot (WB) images are organized in Supplementary Western Blot

Journal: Frontiers in Bioscience-Landmark

Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

doi: 10.31083/fbl26857

Figure Lengend Snippet: Fig. 4. GDF15 decreased oxidative stress in HFpEF. Expression of oxidative stress-associated biomarkers, Myeloperoxidase (MPO) and oxidized low-density lipoprotein (ox-LDL). The original western blot (WB) images are organized in Supplementary Western Blot

Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

Techniques: Expressing, Western Blot

Fig. 5. GDF15 reduces cardiac inflammatory responses in HFpEF. Expression of inflammation-associated biomarkers, Interleukin-

Journal: Frontiers in Bioscience-Landmark

Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

doi: 10.31083/fbl26857

Figure Lengend Snippet: Fig. 5. GDF15 reduces cardiac inflammatory responses in HFpEF. Expression of inflammation-associated biomarkers, Interleukin-

Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

Techniques: Expressing

Fig. 6. GDF15 suppresses apoptosis in cardiomyocytes in HFpEF. (A) Representative photographs of TUNEL staining in heart

Journal: Frontiers in Bioscience-Landmark

Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

doi: 10.31083/fbl26857

Figure Lengend Snippet: Fig. 6. GDF15 suppresses apoptosis in cardiomyocytes in HFpEF. (A) Representative photographs of TUNEL staining in heart

Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

Techniques: TUNEL Assay, Staining